SHP-77人小细胞肺癌细胞(2021年新引进)

产品介绍

目录号 Serial	SCSP-5074
标识符 Identifier	CSTR:19375.09.3101HUMSCSP5074
描述 Description	SHP-77 保留了SCLC的重要特征，具有稳定的生物化学特性，可以连续培养。该细胞系是小细胞肺癌中一种不常见的未分化大细胞突变体。它虽然具有突变体的形态，但保持了经典SCLC的生化特性。电子显微镜观察发现存在腺体形成和胞浆内板层体(intracytoplasmic lamellar bodies)。这些细胞表达神经内分泌标记物L-多巴脱羧酶和致密核心分泌颗粒(dense core secretory granules)。SHP-77可用做检测51Cr和111In释放对体外细胞及裸鼠体内毒性的靶标，用于评估肺癌病患的细胞和血清的免疫反应水平。该细胞可用于评估接受放射或化学疗法治疗的SCLC病患的免疫状况。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X,X；CSF1PO: 10,11；D13S317: 8,8；D16S539: 11,11；D5S818: 12,12；D7S820: 11,11；TH01: 7,7；TPOX: 9,11；vWA: 16,16。
动物种别 Organism	白人
性别 Gender	男性，54岁
组织来源 Tissue and Cell Type	肺; 小细胞肺癌
形态 Morphology	悬浮和松散贴壁
培养基和添加剂 Complete Growth Medium and Culture Conditions	人小细胞肺癌细胞 SHP-77 完全培养液配方(100 ml)： RPMI 1640 Medium (Invitrogen, 11875-093)88 ml FBS (Gibco)10 ml Glutamax (Invitrogen, 35050061) 1 ml Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml 参考传代比例：1:2-1:3 参考传代周期：4-6天参考换液频率：每2-3天换1次液冻存液配方：完全培养液95%，DMSO 5% 【注意事项】该细胞传代时可以轻轻拍打细胞培养瓶侧面十余次，将悬浮的细胞进行收集离心，以40万～50万/ mL的密度重悬传代。轻轻拍打后仍贴壁未悬浮的细胞则丢弃，不收集传代。
供应限制 Permits and Restrictions	A。仅供研究之用。
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