Hep 3B2.1-7人肝癌细胞

产品介绍

目录号 Serial	TCHu106
标识符 Identifier	CSTR:19375.09.3101HUMTCHu106
描述 Description	该细胞系来自8岁男性黑人的组织。其染色体模式数目为60，在裸鼠中能致瘤。已知该细胞系含有与人类疾病有关的因子，需在2级生物安全防护下操作。在本库通过支原体检测。在本库通过STR检测。
动物种别 Organism	人
性别 Gender	男
组织来源 Tissue and Cell Type	肝细胞癌，肝
形态 Morphology	上皮细胞
培养基和添加剂 Complete Growth Medium and Culture Conditions	MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Knowles BB , et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960Knowles BB , Aden DP . Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983Aden DP , et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137Darlington GJ , et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851Cancer Research 63:3775-3782,2003Chinese Journal of Cancer 21(3):267-271, 2002

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