Hep G2 [HEPG2]人肝癌细胞(肝脏)

产品介绍

目录号 Serial	SCSP-510
标识符 Identifier	CSTR:19375.09.3101HUMSCSP510
描述 Description	人肝癌细胞Hep G2 [HEPG2]来源于一个15岁的白人男性肝癌患者。该细胞表达3-羟基-3-甲基戊二酰还原酶(3-hydroxy-3-methylglutaryl-CoA)和肝三酰甘油脂肪(hepatic triglyceride lipase)活性。此细胞不携带乙肝病毒基因组(Hepatitis B virus)。模式染色体数为55(50-60不等)。在免疫抑制小鼠中不致瘤，在半固体培养基中可成瘤。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测结果无误。STR结果如下：D5S818: 11,12；D13S317: 9,13；D7S820: 10,10；D16S539: 12,12；vWA: 17,17；THO1: 9,9；Amelogenin: X，Y；TPOX: 8,9；CSF1PO: 10,11
动物种别 Organism	人
性别 Gender	男性，15岁
组织来源 Tissue and Cell Type	肝脏
形态 Morphology	上皮样，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	人肝癌细胞 Hep G2完全培养液配方(100 ml)： MEM (Invitrogen，11090081) 87 ml FBS (Gibco) 10 ml Glutamax(invitrogen 35050061) 1 ml Non-essential Amino Acids, 100x (Invitrogen, 11140050) 1 ml Sodium Pyruvate 100 mM Solution(invitrogen 11360070) 1 ml 参考传代周期：4-6天参考传代比例：1:3 参考换液频率：传代时换液冻存液：完全培养液95%，DMSO 5% 细胞状态：贴壁生长气相：空气，95%；二氧化碳，5%。温度：37摄氏度。注意事项： 1.Hep G2 [HEPG2]对培养条件要求较严格，特别是pH值和血清质量，否则可能影响细胞凝集数量，应使用高质量低内毒素，未灭火的胎牛血清，可以帮助圆形的细胞丛簇更好的贴壁及形成单层细胞。细胞内容易有空泡，特别是在融合时。 2.该细胞复苏后可能存在形态不典型的情况，经几次传代后，细胞形态会更加典型。 3.该细胞对血清质量较为敏感，我库建议您使用优质胎牛血清进行培养或选择订购我库配套MEM完全培养液，货号为SCSP-651。 4.我库冻存时，每支冻存管约含7×105细胞量，体积为500μl，预期存活率70%，建议复苏至1个T25培养瓶中。
供应限制 Permits and Restrictions	A。仅供研究之用。
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