A549人非小细胞肺癌细胞(2017年新引进)

产品介绍

目录号 Serial	SCSP-503
标识符 Identifier	CSTR:19375.09.3101HUMSCSP503
描述 Description	人非小细胞肺癌细胞A549来源于58岁的白人男性肺癌组织，由D.J. Giard等人建立于1972年。M. Lieber发现A549可以通过胞苷二磷酸胆碱途径合成高比例的不饱和脂肪酸卵磷脂。该细胞复苏后不耐低温和长时间运输，复苏后沿途最低温度需高于10摄氏度且运输路途不可过长。冬天常温邮寄A549细胞时会有细胞脱落、坏死等现象，请尽量选用干冰冷链快递。通过本库支原体检测。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X,Y；CSF1PO: 10,12；D13S317: 11；D16S539: 11,12；D5S818: 11；D7S820: 8,11；THO1: 8,9.3；TPOX: 8,11；vWA: 14
动物种别 Organism	人
性别 Gender	男性，58岁
组织来源 Tissue and Cell Type	肺部
形态 Morphology	上皮样，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	A549细胞完全培养液配方： F-12K(Invitrogen，21127-022) 89 ml FBS(Gibco) 10 ml Glutamax (Invitrogen, 35050061) 1 ml 参考传代周期：每周2-3次参考传代比例：1 : 3 备注:培养时保持细胞密度在6000 到 60000 cells/平方厘米，不要超过70000 cells/平方厘米参考换液频率：每周2-3次冻存液配方：完全培养液95%，DMSO 5% 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
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