ACHN人肾腺癌细胞(2019年新引进)

产品介绍

目录号 Serial	SCSP-5063
标识符 Identifier	CSTR:19375.09.3101HUMSCSP5063
描述 Description	细胞株始建于1979年11月，源于一名22岁的白种人男性恶性胸腔积液，他患有广泛转移的肾腺癌。该细胞的生长受到人干扰素抑制，应用于人干扰素或干扰素诱导物的抗增殖研究。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X；CSF1PO: 11；D13S317: 12；D16S539: 12,13；D5S818: 12；D7S820: 9,11；THO1:8；TPOX: 8,11；vWA: 16,17。
动物种别 Organism	人；白人
性别 Gender	男性，22岁
组织来源 Tissue and Cell Type	肾脏；来源于转移部位：胸腔积液
形态 Morphology	上皮样，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	人肾腺癌细胞ACHN完全培养液配方(100 ml)： MEM (Gibco, 110900081)87 ml Glutamax (Gibco, 35050-061) 1 ml Sodium Pyruvate (Gibco, 11360-070) 1 ml Non-essential Amino Acids, 100 (Invitrogen, 11140050) 1 ml FBS (Gibco)10 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。生物安全等级：BSL-1 参考传代比例：1:2-1:3 参考传代周期：3-5天参考换液频率：每周3次冻存液配方：完全培养液95%，DMSO 5% 【注意事项】该细胞生长受到人类干扰素抑制
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells The ACHN cell line was initiated in November, 1979 from the malignant pleural effusion of a 22-year-old Caucasian male with widely metastatic renal adenocarcinoma (autopsy confirmed). Cells were seeded directly to culture flasks in Eagle's MEM with 10% FBS, then maintained and passaged 150 days in flasks. Cells were then inoculated subcutaneously into nude mice. After 4 weeks, palpable, locally invasive tumors were noted. Both the original cells (ACHN) and those recovered from nude mouse tumors were growth-inhibited by human interferons. Kochevar J. Blockage of autonomous growth of ACHN cells by anti-renal cell carcinoma monoclonal antibody 5F4. Cancer Res. 20: 2968-2972, 1990. PubMed: 2334900

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