产品说明
一般描述
Amino groups are common to many biological compounds and many amines used in pharmaceuticals. Most of these compounds show weak absorption in direct HPLC analysis with UV-VIS detection. However, strongly absorbing derivatives of amines can be easily detected by HPLC with high sensitivity even when present at low concentrations. Primary and secondary amino groups are usually derivatized as aromatic derivatives by nucleophilic substitution reactions using reagents such as arylsulfonyl chlorides.
4-(Dimethylamino)azobenzene-4′-sulfonyl chloride, a well-known UV-labeling agent, is generally used for the derivatization of N-terminal amino acids, imidazole derivatives, polyamines, etc. prior to their chromatographic determination due to the former′s capacity to covalently bind to the substrates. Its derivatives typically absorb UV radiation between the range of 436-460 nm. This method of derivatization is regarded as superior compared to other methods owing to the simplicity of the procedure, high stability and reproducibility, best resolution of the analytes, etc.
应用
4-(Dimethylamino)azobenzene-4′-sulfonyl chloride may be used to derivatize biogenic amines, primary amino acids, melamine, and hydroxyproline and proline extracted from various biological samples prior to their determination using high performance liquid chromatography (HPLC) technique to increase their detection sensitivity.
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Discover LiChropur™ reagents ideal for HPLC or LC-MS analysis
法律信息
LiChropur is a trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 线性分子式 | (CH3)2NC6H4N=NC6H4SO2Cl |
| 分子量 | 323.80 |
| Beilstein | 3064095 |
| EC 号 | 260-235-1 |
| MDL编号 | MFCD00007444 |
产品性质
| 质量水平 | 100 |
| 等级 | for HPLC derivatization |
| 测定 | 98.0-102.0% (AT) |
| 质量 | LiChropur™ |
| technique(s) | HPLC: suitable |
| mp | 185 ℃ (dec.) (lit.) |
| SMILES string | CN(C)c1ccc(cc1)\N=N\c2ccc(cc2)S(Cl)(=O)=O |
| InChI | 1S/C14H14ClN3O2S/c1-18(2)13-7-3-11(4-8-13)16-17-12-5-9-14(10-6-12)21(15,19)20/h3-10H,1-2H3/b17-16+ |
| InChI key | VTVWTPGLLAELLI-WUKNDPDISA-N |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H314 |
| 预防措施声明 | P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| 危险分类 | Skin Corr. 1B |
| 储存分类代码 | 8B - Non-combustible, corrosive hazardous materials |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
