产品说明
一般描述
BIOshell™ IgG 1000 Å C18 is a high-speed, high-performance liquid chromatography column based on a new wide-pore (1000 Å) Fused-Core® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 μm thin porous shell and the small overall particle size of 2.7 μm. The sterically protected C18 bonded phase of BIOshell™ IgG 1000 Å C18 provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds, such as proteins.
应用
UHPLC Analysis of Reduced IgG1 on BIOshell™ IgG 1000 Å C18
UHPLC Analysis of Proteins on BIOshell™ IgG 1000 Å C18, 2.7 μm
UHPLC Analysis of Proteins on BIOshell™ IgG 1000 Å C18, 2.7 μm
UHPLC Analysis of IgG2 on BIOshell™ IgG 1000 Å C4, C18, and Diphenyl, 2.7 μm
分析说明
The target analytes include monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with molecular weights up to 500 kDa.
法律信息
BIOshell is a trademark of sigma-aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
产品性质
| 物料 | stainless steel column |
| agency | suitable for USP L1 |
| 产品线 | BIOshell™ |
| 特点 | endcapped |
| 包装 | pack of 3 ea |
| 标记范围 | 1.4% Carbon loading |
| technique(s) | HPLC: suitable LC/MS: suitable UHPLC-MS: suitable UHPLC: suitable |
| 色谱柱长度 × 内径 | 0.5 cm × 3 mm |
| 基质 | Fused-Core® particle platform superficially porous particle |
| 基质活性基团 | C18 (diisobutyloctadecylsilane) bonding phase |
| 粒径 | 2.7 μm |
| 孔径 | 1000 Å pore size |
| 表面积 | 22 m2/g |
| 操作pH范围 | 1 - 8 |
| 分离技术 | reversed phase |
