产品说明
一般描述
Ascentis® Express 160 Å Phenyl-Hexyl is a high-speed, high-performance liquid chromatography column based on a 160Å Fused-Core® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency for high molecular weight solutes (up to 20 kDa) due to the shallow diffusion paths in the 0.5 μm thick porous shell and the small overall particle size of 2.7 μm. The endcapped, phenyl-hexyl bonded phase provides a stable, reversed phase packing with a pore structure and pore size that is optimized for reversed-phase HPLC separations of peptides and polypeptides, using typical acidic mobile phases favored for proteomic applications.
分析说明
Target analytes include electron-poor molecules, aromatic or unsaturated compounds (ketones, nitriles, alkenes), as well as peptides and polypeptides < 20 kDa.
法律信息
Ascentis is a registered trademark of sigma-aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
产品性质
| 物料 | stainless steel column |
| agency | suitable for USP L11 |
| 产品线 | Ascentis® |
| 特点 | endcapped |
| 包装 | 1 ea of |
| 标记范围 | 4.7% Carbon loading |
| technique(s) | HPLC: suitable LC/MS: suitable UHPLC-MS: suitable UHPLC: suitable |
| 色谱柱长度 × 内径 | 15 cm × 2.1 mm |
| 基质 | Fused-Core® particle platform superficially porous particle |
| 基质活性基团 | Phenyl-Hexyl (dimethylphenyl-hexylsilane) bonding phase |
| 粒径 | 2.7 μm |
| 孔径 | 160 Å pore size |
| 表面积 | 90 m2/g |
| 操作pH范围 | 2 - 9 |
| 分离技术 | reversed phase |
