产品说明
免疫原
Synthetic peptide congugated to protein carrier. The mice were immunized with a combination of N & C peptide. The Swiss Prot ID is Q16531.
应用
For ELISA: Use at an assay dependent dilution. For WB(ECL): Use at a dilution of 1:1,000 in 5% Milk/PBS containing 0.05% Tween. We recommend an overnight incubation at 4¡C. Secondary antibody (GTX30590) 2 hours at RT with dilution of 1:5,000 in 5% Milk/PBS containing 0.05% Tween. Not tested in other applictions. Optiamal dilutions/concentrations should be determined by the researcher.
生化/生理作用
The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells. The injected DDB protein stimulated DNA repair to normal levels in those strains that lacked the DDB activity but did not stimulate repair in cells from XPE patients that contained the activity. These results provided direct evidence that defective DDB activity causes the repair defect in a subset of XPE patients and establishes a role for this activity in nucleotide-excision repair in vivo. It remains for mutation analysis to demonstrate whether the defect in XPE patients is in the DDB1 gene or the DDB2 gene.
特点和优势
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
外形
Phosphate-buffered saline, pH 7.2, containing no preservatives
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 1322 monoclonal |
| 形式 | liquid |
| 分子量 | 130 kDa |
| species reactivity | human |
| 浓度 | 3 mg/mL |
| technique(s) | ELISA: suitable western blot: 1:1000 |
| 同位素/亚型 | IgG |
| UniProt登记号 | Q16531 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... DDB1(1642) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
