产品说明
一般描述
Recombinant, human WEE1 fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. WEE1 is a tyrosine kinase that causes inhibitory phosporylation of Cdk1 and Cdk2 and acts as a mitotic inhibitor. Protects the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 prior to the onset of mitosis.
包装
10 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala,Glu,Lys,Tyr)6:2:5:1 per min at 30°C, using variable concentrations of ATP (100 nM to 1.55 µM).
外形
In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.
重悬
Following initial thaw, aliquot and freeze (-70°C).
其他说明
Katayama, K., et al. 2005. Mol. Cell. Biol.25, 5725.
Yoshida, T., et al. 2004. Ann. Oncol.15, 252.
Watanabe, N., et al. 1995. EMBO J.14, 1878.
Igarashi, M., et al. 1991. Nature353, 80.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 酶学委员会(EC)编号 | 2.7.1.112 ( BRENDA | IUBMB ) |
产品性质
| 形式 | liquid |
| 质量水平 | 100 |
| specific activity | ≥1 units/mg protein |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 运输 | wet ice |
| 储存温度 | −70℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
