产品说明
一般描述
TSK-GEL Octadecyl-4PW 和 Phenyl-5PW RP 色谱柱上较低的 C18 和苯基覆盖率使得其保留能力比硅胶基质的反相色谱柱弱。不建议在涉及小分子量化合物的应用中使用这些色谱柱，但是填料中的大孔(分别为 500Å 和 1000Å)使得它们适用于多肽和蛋白质的分离。1000Å 的超大孔能毫无阻碍地让蛋白质和其他分子质量高达 350,000 道尔顿的生物分子通过。
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
- Octadecyl-2PW particles have 125Å pores
- Octadecyl-4PW particles have 500Å pores
- Phenyl-5PW RP particles have 1000Å pores
- Octadecyl-NPR particles are nonporous
外形
Shipped in 30% methanol - 70% water.
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法律信息
TSKgel is a registered trademark of Tosoh Corporation
产品性质
| 物料 | stainless steel |
| 产品线 | TSKgel® |
| manufacturer/tradename | Tosoh Phenyl-5PW, 08043 |
| 标记范围 | 1 meq/mL ligand density (estimate) |
| 参数 | no limitation salt concentration 0-100% organic solvent 1.2 mL/min flow rate 30 bar max. pressure 4-60 ℃ temperature |
| technique(s) | HPLC: suitable |
| 长度 × 内径 | 7.5 cm × 4.6 mm |
| 基质 | polymer particle platform |
| 基质活性基团 | phenyl phase |
| 粒径 | 10 μm |
| 孔径 | 1000 Å pore diameter |
| 操作pH | 2 - 12 |
| 分离技术 | reversed phase |
