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TSKgel® Phenyl-5PW RP HPLC Column

货号
818756
规格纯度
phase phenyl, L × I.D. 7.5 cm × 2 mm, 10 μm particle size
参考价格
13059.7 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

TSKgel Phenyl-5PW RP columns are filled with 10 micron porous polymethacrylate particles, the surface of which is covered with a high density of phenyl groups that causes proteins to be retained in a low salt buffer mobile phase and requiring a solvent gradient for their elution, as is the protocol in reversed phase chromatography. In contrast, TSKgel Phenyl-5PW has such a low density of phenyl groups on the surface that proteins can only be retained at high salt concentration and eluted by a decreasing salt gradient, which, of course, is the protocol used in hydrophobic interaction chromatography. TSKgel Phenyl-5PW RP columns can withstand rigorous cleaning with either acid or base. The 1000 A pore size accommodates globular protein samples up to 1,000,000 Da.
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.

  • Octadecyl-2PW particles have 125Å pores
  • Octadecyl-4PW particles have 500Å pores
  • Phenyl-5PW RP particles have 1000Å pores
  • Octadecyl-NPR particles are nonporous

外形

Shipped in 30% methanol - 70% water.

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法律信息

TSKgel is a registered trademark of Tosoh Corporation

基本信息

eCl@ss32110501

产品性质

物料stainless steel hardware
产品线TSKgel®
manufacturer/tradenameTosoh Phenyl-5PW, 18756
标记范围1 meq/mL ligand density (estimate)
参数 no limitation salt concentration
0-100% organic solvent
0.12 mL/min flow rate
10 bar max. pressure
4-60 ℃ temperature
technique(s)HPLC: suitable
长度 × 内径7.5 cm × 2 mm
基质 polymer particle platform
基质活性基团phenyl phase
粒径10 μm
孔径1000 Å
操作pH2 - 12
分离技术reversed phase

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