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TSKgel® Chelate-5PW HPLC Column

货号
814440
规格纯度
phase iminodiacetic acid, L × I.D. 5 cm × 5 mm, 10 μm particle size, glass
参考价格
23187.9 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.

Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.

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法律信息

TSKgel is a registered trademark of Tosoh Corporation

产品性质

物料glass
产品线TSKgel®
manufacturer/tradenameTosoh 14440
参数≤20% organic solvent
≤4 M salt concentration
≤45 ℃ temperature
1.0 mL/min flow rate
20 bar max. pressure
technique(s)HPLC: suitable
长度 × 内径5 cm × 5 mm
基质 hydroxylated methacrylic polymer
基质活性基团iminodiacetic acid phase
粒径10 μm
操作pH2 - 12
分离技术affinity

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