产品说明
一般描述
TSKgel Amide-80 columns are packed with 3, 5, or 10 micron spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 ℃) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, the new, high efficiency, 3 micron columns offer shorter analysis time and enhanced sensitivity for LC-MS applications.
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法律信息
TSKgel is a registered trademark of Tosoh Corporation
基本信息
eCl@ss | 32110501 |
产品性质
物料 | stainless steel hardware |
agency | suitable for USP L68 |
产品线 | TSKgel® |
manufacturer/tradename | Tosoh 19697 |
参数 | 0-100% organic solvent 10-80 ℃ temperature 150 bar max. pressure |
technique(s) | HPLC: suitable |
长度 × 内径 | 25 cm × 2 mm |
基质 | silica particle platform |
基质活性基团 | carbamoyl phase |
粒径 | 5 μm |
孔径 | 100 Å |
表面积 | 450 m2/g |
操作pH | 2.0 - 7.5 |
分离技术 | hydrophilic interaction (HILIC) |