产品说明
一般描述
Recombinant, mouse R-Spondin 4 consisting of amino acids 19-228 and expressed in Chinese Hamster Ovary cells. Able to induce activation of β-catenin response in a Luciferase assay using HEK293T human embryonic kidney cells. R-spondin 4 is useful as a positive modulator of Wnt/β signaling.
包装
25 μg in Glass bottle
生化/生理作用
ED₅₀ = 5-20 ng/ml in the presence of 10 ng/ml rmWnt-3a, as measured by induction of activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.
警告
Toxicity: Standard Handling (A)
外形
Lyophilized from 0.2 µm sterile-filtered PBS, NaCl, EDTA, BSA.
重悬
Reconstitute to 200 µg/ml in PBS.
其他说明
Hendrickx, M. and L. Leyns. 2008. Develop. Growth Differ.50, 229.
Ishii, Y., et al. 2008. J. Invest. Dermatol.128, 867.
Kim, K.A., et al. 2008. Mol. Biol. Cell19, 2588.
Binnerts, M.E., et al. 2007. Proc. Natl. Acad. Sci. USA104 14700.
Nam, J.S., et al. 2007. Gene Expr. Patterns7, 306.
Pemberton, T.J., et al. 2007. Dev. Dyn.236, 2245.
Bergmann, C., et al. 2006. Am. J. Hum. Genet.79, 1105.
Blaydon, D.C., et al. 2006. Nat. Genet.38, 1245.
Nam, J.S., et al. 2006. J. Biol. Chem.281, 13247.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
| 测定 | ≥95% (SDS-PAGE) |
| 质量水平 | 100 |
| 形式 | solid |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze |
| 杂质 | <1.0 EU/μg protein Endotoxin |
| 运输 | ambient |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
