产品说明
一般描述
Pwo SuperYield DNA Polymerase is originally isolated from Pyrococcus woesei.
The enzyme accepts modified nucleotides for efficient labeling of nucleic acids by PCR.
PCR products are blunt-ended directly useable for blunt-end ligation.
Using the magnesium-containing reaction buffer supplied, the final MgCl2 concentration is 1.5mM.
The core component of the Pwo Master is the proofreading Pwo SuperYield DNA Polymerase which has both polymerase activity and a 3′–5′ exonuclease (proofreading) activity. Pwo SuperYield DNA Polymerase combines Pwo DNA Polymerase with an optimized buffer system. This buffer system enhances the enzymatic properties of the enzyme, resulting in higher yields of the amplification without changing the fidelity of DNA synthesis.
应用
Use this premixed, double-concentrated formulation containing PwoSuperYield DNA Polymerase, a new optimized buffer system, and PCR grade nucleotides, if your application requires better fidelity than provided by (FastStart) Taq DNA Polymerase. The product can be applied for:
- Accurate amplification of DNA critical applications
- High-throughput PCR
- High fidelity PCR
- Site-directed mutagenesis
- Cloning
- Gene expression
特点和优势
Pwo Master is a ready-to-use, 2x concentrated master mix. Pwo SuperYield DNA Polymerase has polymerase and 3′ → 5′ exonuclease proofreading activity, including an optimized buffer. The buffer enhances enzyme performance, synthesizing higher DNA yields of high fidelity.
- Higher yield and 18-fold higher fidelity
- Maximize convenience.
- Reduce setup time.
包装
1 kit containing 2 components
质量
Quality control is performed using human genomic DNA, with primers specific for tPA that generate a 1.7kb product.
单位定义
Volume Activity: 2.5.U - 50 μl reaction
其他说明
仅用于生命科学研究。不可用于诊断。
法律信息
Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
产品性质
| 质量水平 | 100 |
| 用途 | sufficient for ≤100 reactions |
| 包装 | pkg of 2.5 mL (10 x 250 μl) |
| manufacturer/tradename | Roche |
| 参数 | 72 ℃ optimum reaction temp. |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
组份列表
组份不可单独销售
| 货号 | 组份 |
| Master Mix, containing Pwo SuperYield DNA Polymerase in reaction buffer 2× concentrated | |
| Water, PCR Grade |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | does not flash |
| 闪点(C) | does not flash |
