产品说明
一般描述
Recombinant λ PPase expressed in E. coli. A 221-amino acid, Mn2+-dependent, protein phosphatase. Hydrolyzes phosphate groups on serine, threonine, tyrosine, or histidine residues. Inhibited by orthovanadate. Note: 1KU = 1,000 units.
包装
20 ku in Plastic ampoule
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per min at 30°C, pH 7.8. 20-200 Units will typically remove >95% of phosphates from serine/threonine residues in 0.1 nmol of protein in 30 min. 100-1000 Units will typically remove >95% of phosphates from tyrosine in 0.1 nmol of protein in 30 min.
外形
In 100 mM NaCl, 50 mM HEPES, 2 mM DTT, 0.1 mM MnCl₂, 0.1 mM EGTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5.
制备说明
Further dilute in 50 mM Tris-HCl, pH 7.8, 5 mM DTT, 2 mM MnCl₂, and 100 µg/ml BSA.
重悬
Following initial thaw, aliquot and freeze (-70°C).
其他说明
Zhuo, S., et al. 1993. J. Biol. Chem.268, 17754.
Gordon, J.A. 1991. Methods Enzymol.201, 477.
Cohen, P.T.W. and Cohen, P. 1989. Biochem. J.260, 931.
法律信息
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
| 质量水平 | 100 |
| 重组 | expressed in E. coli |
| 测定 | ≥95% (SDS-PAGE) |
| 形式 | liquid |
| specific activity | ≥400,000 units/mL |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 异质活性 | Protease, none detected |
| 运输 | wet ice |
| 储存温度 | −70℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
