产品说明
一般描述
The PCR ELISA, DIG-Detection kit is used for semi-quantitative nonradioactive detection of polymerase chain reaction (PCR) products using a convenient microplate format. We recommend to use the PCR ELISA, DIG-Detection, 5-pack kit in combination with the PCR ELISA, DIG-Labeling kit for specific DNA analysis.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
特异性
The high specificity of digoxigenin detection permits distinguishing specific and nonspecific amplification products. This is due to the hybridization step with the capture probe. The specificity of this hybridization follows the standard rules of hybridization assays. Stringent hybridization in the digoxigenin detection depends on the length and the GC content of the capture probe and the incubation temperature. Temperatures up to 55 ℃ are compatible with digoxigenin detection. Under these conditions, with short capture probes (18 to 20mers), identification of a single base mismatch in the capture oligonucleotide is possible. For this reason, mutations in template DNAs can also be detected using DIG.
应用
This kit is used in research studies for the semi-quantitative detection of digoxigenin-labeled PCR products. It is particularly suitable for the parallel testing of a large number of samples.
Note: The PCR ELISA system provides a convenient, nonradioactive method for labeling and detecting PCR products. It is approximately 100 times more sensitive than conventional ethidium bromide staining of products on agarose gels.
特点和优势
This kit is part of a complete PCR ELISA system. If you combine it with other reagents and equipment, you can adapt the system for many molecular biology applications. For example:
- If you have an appropriate target-specific, biotin-labeled capture probe, you can distinguish PCR products that differ by as little as a single base pair. Thus the PCR ELISA system (DIG-labeling + DIG-detection) can be used:
- To detect point mutations, deletions or insertions.
- To classify target sequences (e.g., as in HLA-typing or cell typing).
- Preparation of suitable DIG-labeled standards and addition of a colorimetric detection system will allow you to quantify PCR products.
- The microplate format used by the PCR ELISA system makes the system compatible with automated plate preparation and reading systems.
Capacity: This size of the PCR ELISA, DIG-Detection kit allows semi-quantitative detection of more DIG-labeled PCR products. The total number of PCR products detected will vary depending upon the number of sample dilutions, controls, and/or standards used in the assay.
包装
1 kit containing 9 components.
原理
Step 1: Amplification of DNA in the presence of digoxigenin-11-dUTP (DIG-dUTP) to generate DIG-labeled PCR product.
Step 2: Denaturation of PCR product and hybridization to a biotin-labeled capture probe. The capture probe specifically recognizes an internal sequence in the target DNA.
Step 3: Immobilization of biotin-labeled hybrid on a streptavidin-coated microplate, followed by plate washes to remove unhybridized, non-specific amplification products.
Step 4: Detection of bound hybrids with peroxidase-conjugated anti-digoxigenin antibody (Anti-DIG-POD) and the colorimetric peroxidase substrate, ABTS.
Results can be measured with a standard microplate reader.
其他说明
仅用于生命科学研究。不可用于诊断。
产品性质
| 质量水平 | 100 |
| 用途 | sufficient for 480 detection reactions (five microplates) |
| 包装 | kit of 1 (9 components) |
| manufacturer/tradename | Roche |
| 环保替代产品特性 | Designing Safer Chemicals Learn more about the Principles of Green Chemistry. |
| application(s) | detection |
| 环保替代产品分类 | Aligned |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
组份列表
组份不可单独销售
| 货号 | 组份 |
| Denaturation Solution for standard protocol | |
| Denaturation Solution for alternate protocol | |
| Hybridization Buffer | |
| Washing Buffer Tablets | |
| Conjugate Dilution Buffer | |
| Anti-Digoxigenin-Peroxidase Conjugate antibody, lyophilizate | |
| ABTS Solution | |
| StreptaWell, microplate modules (8 wells each) in a strip frame | |
| Adhesive Plate Cover Foils |
安全信息
| 象形图 |   |
| 警示用语： | Warning |
| 危险声明 | H317 - H411 |
| 预防措施声明 | P261 - P273 - P280 - P333 + P313 - P362 + P364 - P391 |
| 危险分类 | Aquatic Chronic 2 - Skin Sens. 1 |
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | does not flash |
| 闪点(C) | does not flash |
