产品说明
一般描述
The MISSION TRC3 ORF GFP Control Vector is a lentivirus plasmid vector. The vector contains an ORF insert expressing TurboGFP™, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format, including our latest Whole Genome LentiORF Pool. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
This positive control can be used for measuring transduction efficiency and optimizing ORF delivery in cell lines that do not survive any puromycin exposure. The GFP Control contains a gene encoding TurboGFP driven by the EF1a promoter. This control provides fast visual confirmation of successful transduction. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively.
In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The MISSION TRC3 ORF GFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
应用
Functional Genomics/Overexpression Screening/Target Validation
法律信息
Use of this product is subject to one or more license agreements. For details, please see the MISSION License Page
MISSION is a registered trademark of sigma-aldrich Co. LLC
TurboGFP is a trademark of Evrogen Co.
基本信息
| NACRES | NA.51 |
产品性质
| 包装 | vial of 20 μL |
| 质量水平 | 200 |
| 浓度 | 500 ng/μL |
| application(s) | genome mapping genomic analysis |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
