产品说明
一般描述
Sigma′s Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Each miRNA construct has been cloned and sequence verified. Mature microRNA sequences are obtained from miRBase.
Lentiviral transduction particles are produced from sequence-verified lentiviral plasmid vectors. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. The polymerase II promoter, elongation factor 1 alpha (EF1A), was chosen to drive miRNA expression needed for reverse transcription of viral RNA and integration of viral DNA into the host cell genome. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory element allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells. Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific miRNA construct into differentiated and non-dividing cells, such as neurons and dendritic cells.
其他说明
Based on miRBase V20 Mature ID
推荐产品
Two negative controls are available: NCLMIR001 and NCLMIR002
法律信息
MISSION is a registered trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.51 |
产品性质
| 质量水平 | 200 |
| 产品线 | MISSION® |
| 浓度 | ≥1x106 VP/ml (via p24 assay) |
| 成熟序列 | UUAGGCCGCAGAUCUGGGUGA |
| Sanger mature/minor 登记号 | MIMAT0005885 |
| Sanger microRNA登记号 | MI0006357 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
