位置:首页 > 品牌 > Sigma-Aldrich >

MISSION® Lenti microRNA, Human

品牌
Sigma-Aldrich
货号
HLMIR0165
包装型号
规格纯度
hsa-miR-1295a
参考价格
7541.62 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Sigma′s Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Each miRNA construct has been cloned and sequence verified. Mature microRNA sequences are obtained from miRBase.

Lentiviral transduction particles are produced from sequence-verified lentiviral plasmid vectors. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. The polymerase II promoter, elongation factor 1 alpha (EF1A), was chosen to drive miRNA expression needed for reverse transcription of viral RNA and integration of viral DNA into the host cell genome. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory element allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells. Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific miRNA construct into differentiated and non-dividing cells, such as neurons and dendritic cells.

其他说明

Based on miRBase V20 Mature ID

推荐产品

Two negative controls are available: NCLMIR001 and NCLMIR002

法律信息

MISSION is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRESNA.51

产品性质

质量水平200
产品线MISSION®
浓度≥1x106 VP/ml (via p24 assay)
成熟序列UUAGGCCGCAGAUCUGGGUGA
Sanger mature/minor 登记号MIMAT0005885
Sanger microRNA登记号MI0006357
运输dry ice
储存温度−70℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号