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MISSION® Lenti microRNA Inhibitor, Human

品牌
Sigma-Aldrich
货号
HLTUD0773
包装型号
规格纯度
hsa-miR-552
参考价格
7541.62 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Individual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. The lentiviral microRNA Inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) is included, allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells.

  • Allows for potent inhibition of the desired miRNA
  • Lentiviral delivery format allows for efficient delivery of the inhibitor into a wide variety of cell types
  • Enables long-term inhibition without repeat transfection

其他说明

Based on miRBase V19 Mature ID

法律信息

MISSION is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRESNA.51

产品性质

质量水平200
产品线MISSION®
浓度≥1x106 VP/ml (via p24 assay)
technique(s)capture ELISA: 106 TU/mL using p24 (Volume 200 uL)
成熟序列AACAGGUGACUGGUUAGACAA
Sanger mature/minor 登记号MIMAT0003215
Sanger microRNA登记号MI0003557
运输dry ice
储存温度−70℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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