产品说明
一般描述
KAPA Taq HotStart® with dNTPs is supplied in a 2X ReadyMix™ format containing all the components required for PCR except primers and template-simply use PCR-grade water to make up the required reaction volume. KAPA Taq ReadyMix is also available with loading dye reaction buffer, allowing, you to load your PCR product directly onto the agarose gel with no extra steps for adding loading/tracking dye. KAPA Taq DNA Polymerase is the single-subunit Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus, purified from recombinant Escherichia coli.
应用
KAPA Taq HotStart® has been used for:
- High throughput PCR
- Amplification of low copy DNA templates
- Multiplex PCR
- Specific amplification of complex templates
- RT-PCR
- Aquarium DNA extraction to generate marker gene libraries
生化/生理作用
KAPA Taq HotStart® DNA polymerase and KAPA Taq exhibit 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The enzyme shows an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity. KAPA Taq hotstart buffer is formulated uniquely to facilitate specific primer annealing. This translates to higher yields of the specific product when compared to traditional Taq buffers. It also enhances the amplification of GC- and AT-rich templates. KAPA Taq hotstart DNA polymerase might be used in combination with any standard Taq buffer with a pH of 8.3 or higher
特点和优势
High performance
- Improved sensitivity, specificity, and yields.
- Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product.
Quick Notes:
- KAPA Taq HotStart® DNA Polymerase can replace any commercial hotstart Taq DNA polymerase in an existing protocol. The final MgCl2 concentration and annealing temperature may need to be optimized to account for differences in formulation.
- The KAPA Taq HotStart Buffer is a uniquelyformulated buffer offering improved specificity and sensitivity, and improved amplification of GC- and AT-rich templates.
- The KAPA Taq HotStart Buffer does not contain MgCl2; MgCl2 (25 mM) is supplied separately to allow greater flexibility during reaction setup.
- The KAPA Taq HotStart PCR Kit is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.
质量
Each batch of KAPA Taq HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq HotStart PCR kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
制备说明
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4℃ for short-term use (up to 1 month). Return to -20℃ for long term storage.
其他说明
For Research Use Only. Not for use in diagnostic procedures.
法律信息
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of sigma-aldrich Co. LLC
基本信息
NACRES | NA.55 |
产品性质
质量水平 | 100 |
用途 | sufficient for 1000 reactions sufficient for 500 reactions |
保质期 | ≤18 mo. |
特点 | dNTPs included hotstart |
包装 | pkg of 250 U (KK1509) pkg of 500 U (KK1511) |
manufacturer/tradename | Roche |
technique(s) | PCR: suitable |
input | purified DNA |
运输 | dry ice |
储存温度 | −20℃ |
组份列表
组份不可单独销售
货号 | 组份 |
KAPA Taq HotStart® DNA Polymerase 5 U/µL | |
5X KAPA Taq HotStart® Buffer | |
MgCl2 25 mM | |
KAPA dNTP Mix 10 mM each |
安全信息
象形图 | |
警示用语: | Warning |
危险声明 | H302 - H371 |
预防措施声明 | P260 - P264 - P270 - P301 + P312 + P330 - P308 + P311 - P501 |
危险分类 | Acute Tox. 4 Oral - STOT SE 2 |
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | does not flash |
闪点(C) | does not flash |