产品说明
一般描述
HiLoad® 16/600 Superdex® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice for separating MAbs from dimers and contaminants.
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
特点和优势
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
适用性
All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography, e.g, separate mAbs from dimers and contaminants
储存及稳定性
Store at 4 to 30 ℃ (20% Ethanol)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
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法律信息
HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva
基本信息
| NACRES | SB.52 |
产品性质
| 物料 | bed support (polyamide) glass column glass fiber (polypropylene reinforced) |
| 保质期 | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| 分子量 | 10000-600000 Da (globular proteins) |
| 包装 | pkg of 1 ea |
| manufacturer/tradename | Cytiva 28-9893-35 |
| 参数 | <1.0 mL/min flow rate 0.3 Mpa (42 psi) |
| 柱床体积 | 120 mL |
| 色谱柱长度 × 内径 | 60 cm × 16 mm |
| 样品体积 | <5 mL |
| 基质 | agarose dextran composite dextran-agarose particle platform polymeric |
| 基质活性基团 | dextran-agarose phase |
| 平均零件尺寸 | 34 μm |
| working range | 3-12 |
| 清洗 | 1 - 14 |
| 分离技术 | size exclusion (SEC) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H226 |
| 预防措施声明 | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| 储存分类代码 | 3 - Flammable liquids |
