产品说明
一般描述
Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide. Optimum pH: 5.8.
Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.
包装
50 μL in Plastic ampoule
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.
外形
Sterile-filtered in 20 mM Tris-HCl, pH 7.5.
重悬
There is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.
其他说明
Murata, T., et al. 2003. Eur. J. Biochem.270, 3709.
Scudder, P., et al. 1986. Eur. J. Biochem.157, 365.
Scudder, P., et al. 1984. J. Biol. Chem.259, 6586.
Scudder, P., et al. 1983. Biochem. J.213, 485.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
酶学委员会(EC)编号 | 3.2.1.103 ( BRENDA | IUBMB ) |
产品性质
质量水平 | 100 |
重组 | expressed in E. coli |
形式 | liquid |
specific activity | ≥14 U/mL ≥140 units/mg protein |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |