DNA Molecular Weight Marker VII

产品说明

一般描述

Contents
Ready-to-use solution, 250 μg/ml, in TE buffer (Tris-HCl, 1 mM EDTA, pH 8.0).
Fragment mixture prepared by cleavage of SPP1-DNA with restriction endonuclease EcoR I.
The product is also available as DNA Molecular Weight Marker VII, Digoxigenin-labeled.
Size Range: 0.081 to 8.57 kbp

应用

DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:

• Restriction digests
• PCR
• RT-PCR

序列

The mixture contains seventeen fragments with the following base pair lengths (1 base pair = 660 daltons): 81, 359, 492, 710, 718*, 992, 1164, 1482, 1515, 1882, 1953, 2799, 3639, 4899, 6106, 7427, and 8576 as determined by computer analysis of the SSP1 sequence.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
*Alonso, J.C. and Trautner, T.A., personal communication of unpublished results, the 718-bp fragment is present in non-stoichometric amounts.

单位定义

50 μg = 1A₂₆₀ unit

制备说明

Storage conditions (working solution): 2 to 8 ℃
Once thawed store at 2 to 8 ℃.

其他说明

仅用于生命科学研究。不可用于诊断。

产品性质

安全信息