DNA Molecular Weight Marker V

产品说明

一般描述

Fragment mixture prepared by cleavage of pBR322 DNA with restriction endonuclease Hae III.

Size Range: 8 to 587 bp

应用

DNA Molecular Weight Marker V is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:

<UL><LI>Restriction digests</LI>

<LI>PCR</LI>

<LI>RT-PCR</LI></UL>

组分

Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0)

序列

The mixture contains 22 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 8, 11, 18, 21, 51, 57, 64, 80, 89, 104, 123, 124, 184, 192, 213, 234, 267, 434, 458, 504, 540, and 587 as determined by computer analysis of the pBR322 sequence.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

单位定义

50 μg = 1A₂₆₀ unit

制备说明

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents

Working concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG).

Storage conditions (working solution): 2 to 8 ℃
Once thawed we recommend further storage at 2 to 8 ℃.

储存及稳定性

Store at -15–-25 ℃. (until use)

其他说明

仅用于生命科学研究。不可用于诊断。

产品性质

安全信息