产品说明
一般描述
Fragment mixture prepared by cleavage of equimolar amounts of λDNA and pSPTBM20 DNA with restriction endonucleases Sty I and Sau I.
Ready-to-use solution, 250 μg/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).
Size Range: 0.07 to 19.3 kbp
应用
DNA Molecular Weight Marker IV is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:
- Restriction digests
- PCR
- RT-PCR
序列
The mixture contains 14 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 74, 421, 697, 925, 1150, 1489, 1882, 2322, 2690, 3140, 4254, 5526, 7743, and 19329 as determined by computer analysis of λDNA and pSPTBM 20 sequences.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
制备说明
Storage conditions (working solution): 2 to 8 ℃
Once thawed, store at 2 to 8 ℃.
其他说明
仅用于生命科学研究。不可用于诊断。
产品性质
| 形式 | solution |
| 质量水平 | 100 |
| 包装 | pkg of 50 μg (in 200 μl) |
| manufacturer/tradename | Roche |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | does not flash |
| 闪点(C) | does not flash |
