产品说明
一般描述
dGTP is a 100 mM clear, colorless solution of the lithium salt (pH 7).
dGTP solution in water (pH 7), 100 mM, lithium salt.
应用
2′-脱氧鸟苷5′-三磷酸三钠盐可用于DNA测序、PCR和其他基于DNA聚合酶的DNA合成和修复技术。
Use dGTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
生化/生理作用
2′-脱氧鸟苷5′-三磷酸可用于体内合成，通过DNA聚合酶合成DNA。
质量
Typical analysis:
- ≥98% dGTP (HPLC), ≤1.5% dGDP (HPLC), ≤0.5% dGMP (HPLC)
- Absorbance determination A250/A260, A280/A260, A290/A260
- DNase activity negative, according to the current Quality Control procedures
- Suitability for PCR
单位定义
1 μmol dGTP = 13.7 A252 units
其他说明
For life science research only. Not for use in diagnostic procedures.
Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
- nucleotide sets (all 4 nucleotides in separate vials)
- ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)
They are also vital components of:
- kits (dNTPs, buffer, and polymerase in separate vials)
- ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]
基本信息
| 经验(实验)分子式 | C10H13N5Na3O13P3 |
| 分子量 | 573.13 |
| MDL编号 | MFCD00080337 |
| PubChem化学物质编号 | 329749301 |
产品性质
| 质量水平 | 100 |
| 描述 | Lithium salt |
| 测定 | =98% |
| 形式 | solution |
| 用途 | sufficient for 6,250 standard reactions (PCR) |
| 包装 | pkg of 250 μL (25 μmol; 100 mM) |
| manufacturer/tradename | Roche |
| 浓度 | 100 mM |
| technique(s) | DNA amplification: suitable DNA sequencing: suitable DNA synthesis: suitable PCR: suitable |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| SMILES string | [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1 |
| InChI | 1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1 |
| InChI key | IWGGLKOTEOCWQP-BIHLCPNHSA-K |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | No data available |
| 闪点(C) | No data available |
