产品说明
一般描述
The Cell Viability Imaging Kit is a simple and rapid way to measure mammalian cell viability using fluorescence microscopy and automated imaging platforms, such as the Cellavista System. Optimized for 96-well plates, the numbers of viable and dead cells, as well as total cell numbers, are determined simultaneously for the area of the well selected.
The kit is designed for 5 × 96 reactions for use in medium to high throughput analysis of cell behavior.
- Determine total cell number using fluorescence staining of cell nuclei.
- Fluorimetrically determine the percentage of dead cells based on membrane permeability.
- Verify the labeling of viable cells quantified by fluorescence staining using a vital dye
应用
Cell quantification and cell viability control are very important methods in cell biology. This includes both life science research and industrial applications. The simple, accurate and fast “one step assay” makes the Cell Viability Imaging Kit an ideal tool for quality control of cells in cellular workflows, especially for medium to high throughput applications.
特点和优势
Cell counting and cell viability are essential for cellular workflows. The Roche Cell Viability Imaging Kit meets the following requirements:
- No risk of losing cells in the one step assay: There are no fixation or washing steps.
- Save time and resources: Total assay time for a 1 x 96 well microplate is only 35 minutes.
- Obtain highly reproducible results using automated imaging and data evaluation with Roche′s Cellavista System.
包装
1个试剂盒包含3种组分。
制备说明
Storage conditions (working solution): Vial 3: The product is stable until the expiration date printed on the label when stored at -15 to -25 ℃. Contents can be frozen and thawed at least 5 times after preparation of solution.
储存及稳定性
Store at -15–-25 ℃. (Vial 1 and Vial 2 can be frozen and thawed at least 5 times. Vial 3 can be frozen and thawed at least 5 times after preparation of solution.)
其他说明
仅用于生命科学研究。不可用于诊断。
产品性质
质量水平 | 100 |
用途 | sufficient for 5 96-well plate(s) |
包装 | pkg of 1 kit |
manufacturer/tradename | Roche |
储存条件 | protect from light |
荧光 | λex 361 nm; λem 486 nm (nuclei dye) λex 490 nm; λem 515 nm (viable dye) λex 535 nm; λem 617 nm (dead dye) |
detection method | fluorometric |
运输 | dry ice |
储存温度 | −20℃ (−15℃ to −25℃) |
组份列表
组份不可单独销售
货号 | 组份 |
Nuclei Dye (blue cap) for fluorimetric determination of cell count based on labeling of cell nuclei | |
Dead Dye (red cap) for fluorimetric determination of dead cells based on cell permeability | |
Viable Dye ( green cap) for fluorimetric determination of viable cells based on metabolic activity |
安全信息
象形图 | |
警示用语: | Warning |
危险声明 | H341 |
预防措施声明 | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
危险分类 | Muta. 2 |
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |