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Bln I (Avr II)

品牌
Roche
货号
BLNI-RO
规格纯度
from Brevibacterium linens
参考价格
2113.9 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Bln I recognizes the sequence C↓CTAGG and generates fragments with 5′-cohesive termini.

Compatible ends
Bln I ends are compatible with ends generated by Nhe I, Spe I and Xba I.

Isoschizomers
Bln I is an isoschizomer of Avr II.
Note: The complete 13 site Avr II restriction map of the E.coli genome has been reported.

Methylation sensitivity
The enzyme is not known to be affected by methylation.

特异性

Recognition sites: CCTAGG
CCTAGG
Restriction site: C↓CTAGG
C↓CTAGG
Heat inactivation: No inactivation of Bln I after incubation at 65 ℃ for 15 minutes.

质量

Absence of nonspecific endonuclease activities
1 μg λDNA is incubated for 16 hours in 50 μl SuRE/Cut Buffer H with an excess of Bln I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.

Absence of exonuclease activity
Approximately 5 μg [3H] labeled calf thymus DNA are incubated with 3 μl Bln I for 4 hours at +37℃ in a total volume of 100 μl 50 mM Tris-HCl, 10 mM MgCl2, 1 mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.

Typical ligation and recutting assay
Bln I fragments obtained by complete digestion of 1 μg λ × EcoR I DNA ligated for 16 hours at +4℃ with 1 U T4 DNA Ligase in 10 μl buffer that contains 66 mM Tris-HCl, 5 mM MgCl2, 5 mM Dithiothreitol, 1 mM ATP, pH 7.5 (at +20℃). The percentages of product that can be ligated and subsequently recut with Bln I and EcoR I (yielding the typical pattern of λ × EcoR I × Bln I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.

DNA图谱分析

Number of cleavage sites on different DNAs

  • λ: 2
  • φX174: 0
  • Ad2: 2
  • M13mp7: 0
  • M13mp18:0
  • pBR322: 0
  • pBR328: 0
  • pUC18: 0
  • SV40: 2

单位定义

One unit is the enzyme activity that completely cleaves 1 μg λ x EcoR I DNA fragments in one hour at +37 ℃ in a total volume of 25 μl (1x) SuRE/Cut Buffer H.

分析说明

SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity

  • A: 25-50%
  • B: 50-75%
  • H: 100%
  • L: 0-10%
  • M: 25-50%


Activity in PCR buffer: Not tested
PFGE tested
Bln I has been tested in Pulsed-Field Gel Electrophoresis (on bacterial chromosomes). For cleavage of genomic DNA (E.coli C 600) embedded in agarose for PFGE analysis, we recommend using 10 U of enzyme/μg DNA and 4 hour incubation.

其他说明

仅用于生命科学研究。不可用于诊断。

产品性质

质量水平100
生物来源bacterial (Brevibacterium linens)
形式solution
包装pkg of 1,000 U (11558170001 [10 U/μl])
pkg of 200 U (11558161001 [10 U/μl])
manufacturer/tradenameRoche
参数37 ℃ optimum reaction temp.
运输dry ice
储存温度−20℃

组份列表

组份不可单独销售

货号组份
Enzyme Solution
SuRE/Cut Buffer H 10x concentrated

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)does not flash
闪点(C)does not flash

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