产品说明
一般描述
D-galactose:NAD+ 1-oxidoreductase
特异性
Galactose dehydrogenase will oxidize D-galactose [Km = 0.7 mM (30 ℃, pH 9.1); relative rate = 1.00], L-arabinose (relative rate = 0.47), D-fucose (relative rate = 1.05), 2-deoxy-D-galactose (relative rate = 0.27), 2-amino-2-deoxy-D-galactose [galactosamine], (relative rate = 0.02; pH 8.6).
Galactose dehydrogenase does not oxidize L-galactose, D-arabinose, D-glucose, L-fucose, D-ribose, D-xylose, D-glucuronic acid, D-galacturonic acid or D-galactose-6-phosphate. The enzyme will use either NAD (Km = 0.24 mM; relative rate = 1.0) or NADP (Km = 2.3 mM; relative rate = 0.32).
质量
Contaminants: <0.01% ADH, <0.01% ?-galactosidase, <0.1% NADH oxidase, <0.5% LDH
序列
Galactose dehydrogenase from Pseudomonas fluorescens (MW 64,000 D) is a dimer with subunits of equal size (subunit MW = 32,000 D).
单位定义
One unit galactose dehydrogenase will oxidize 1 mol of β-D-galactose in one minute at +25 ℃ and pH 8.6.
The QC assay produces 1 mol of NADH per mol of galactose oxidized.
外形
Suspension in 3.2 M ammonium sulfate solution, 1 mM EDTA, pH approximately 6
其他说明
仅用于生命科学研究。不可用于诊断。
基本信息
| 酶学委员会(EC)编号 | 1.1.1.48 ( BRENDA | IUBMB ) |
产品性质
| 质量水平 | 100 |
| 生物来源 | Pseudomonas fluorescens |
| 形式 | suspension |
| specific activity | ~5 units/mg protein (At 25 ℃ with D-galactose as the substrate; standardized with BSA.) |
| 包装 | pkg of 1 mL (10104973001 [1 mg]) pkg of 1 mL (10104981001 [5 mg]) |
| manufacturer/tradename | Roche |
| 最佳pH | 8.4 (0.1 M in Tris buffer) |
| pH范围 | 4.6 - 9.2 |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | No data available |
| 闪点(C) | No data available |
