Anti-Uncoupling Protein 1 Antibody

产品说明

特异性

Uncoupling Protein 1 (UCP1), mouse. Mouse/rat UCP1 is a 307 amino acid mitochondrial uncoupling protein (Bouillaud et al. 1986; Ridley et al. 1986; Kozak et al. 1988; Cassard et al. 1990; Miroux et al. 1993). It is only 59% homologous with UCP2 (Fleury et al. 1997). Human UCP1 is located on chromosome 4 (Bouillaud et al. 1986; Ridley et al. 1986; Kozak et al. 1988; Cassard et al. 1990; Miroux et al. 1993). UCP1 is primarily expressed in brown adipose tissues.

The antibody has not yet been tested for cross-reactivity to other species. The sequence used for immunization shows 100% homology to rat, 91% with golden hamster, 83% with rabbit and 75% with human and canine UCP1.

免疫原

A 12 aa peptide at the C-terminus of the mouse/rat UCP1 (Bouillaud et al. 1986; Ridley et al. 1986; Kozak et al. 1988; Cassard et al. 1990; Miroux et al. 1993).

应用

Detect Uncoupling Protein 1 using this Anti-Uncoupling Protein 1 Antibody validated for use in ELISA & WB.
Western Blotting: 1-10 μg/mL using Chemiluminescence technique. An antibody made to this epitope has detected a protein of ~32 kDa in brown adipose tissues (Feil & Rafael 1994).

ELISA: 1:10,000-1:100,000; using 50-100 ng UCP1 control peptide/well.

Optimal working dilutions must be determined by end user.

外形

Affinity purified immunoglobulin. Liquid in PBS with less than 0.1% sodium azide.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息