产品说明
一般描述
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
特异性
Expected to cross-react with mammals, sea urchin, plants, not yeast (Paturle-Lafanechere, L., 1991).
The antibody specifically recognizes the detyrosinated form (Glu tubulin) of the tubulin alpha chain (Bianchi, M., 2006; Goulimari, P., 2005).
免疫原
Peptide corresponding to the C-terminal of Glu tubulin.
应用
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Anti-Tubulin Antibody, Detyrosinated is an antibody against Tubulin for use in IC, IH(P) and WB.
Immunohistochemistry:
A previous lot was used on either frozen or paraffin embedded, fixed tissue (Gundersen, G.G., 1984): 1:200-1:500 dilution.
Optimal working dilutions must be determined by the end user.
质量
Routinely evaluated by Western Blot on L6 lysate lysates.
Western Blot Analysis: 1:500 dilution of this lot detected detyrosinated tubulin on 10 μg of L6 lysate.
目标描述
50 kDa
外形
Purified rabbit polyclonal in buffer containing liquid PBS.
Affinity purified immunoglobulin
储存及稳定性
Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
Hela Whole Cell Lysate.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | rat, human, sea urchin, plant, mouse |
should not react with | Saccharomyces cerevisiae |
species reactivity (predicted by homology) | mammals |
manufacturer/tradename | Chemicon® |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable (paraffin) western blot: suitable |
NCBI登记号 | NM_006086.2 |
UniProt登记号 | Q13509 |
运输 | dry ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |