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Anti-Podocalyxin-like protein I (Cytotoxic) Antibody, clone mAb 84

品牌
Sigma-Aldrich
货号
MABD89
规格纯度
clone mab 84, from mouse
参考价格
5854.74 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Podocalyxin-like protein 1 (PODXL) is a transmembrane protein known to be heavily sialyated and sulfated that is localized on the apical surface of podocytes and on vascular endothelia. Podocalyxin protein is expressed on the surface of undifferentiated human embryonic stem cells. Originally, the protein was characterized as a marker for vasculature and podocytes, but expression has since been observed in platelets, hematopoietic precursor cells and mesothelial cells from the coelmic cavity lining. Studies have outlined two distinct and seemingly adverse roles for this protein; it acts as an anti-adhesion molecule in renal glomerulae podocytes, but functions as an adhesion molecule within vasculature on high endothelial venules. Podocalyxin expression has been implicated in the development of aggressive cancers, beta catenin signaling, angiogenisis, and both myeloid and acute lymphoblastic leukemias.

特异性

This antibody recognizes the extracellular domain of Podocalyxin-like protein 1.
This antibody has been shown to select against undifferentiated human embryonic stem cells (hESC) and to be functionally cytotoxic against these undifferentiated hESC populations (Choo, A., et al., 2008).

免疫原

HES-3 Human embryonic stem cell line

应用

Anti-Podocalyxin-like protein I (Cytotoxic) Antibody, clone mAb 84 is an antibody against Podocalyxin-like protein I (Cytotoxic) for use in Western Blotting, ICC, Flow Cytometry.
This antibody was shown to induce cell death of undifferentiated, but not differentiated hESC, within 30 minutes of incubation (Choo, A., et al. (2008). Stem Cells 26:1454-1463). This antibody has been shown to work in Western Blot applications (Choo, A., et al. (2008). Stem Cells, 26:1454–1463).
This antibody has been shown to work in Flow Cytometry applications (Choo, AB., et al. (2008). Stem Cells, 26:1454–1463).

质量

Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Podocalyxin-like protein I in H9 human embryonic stem cells.

目标描述

55 kDa calculated

联系

Replaces: MAB4414

外形

Purified by Ion-Exchange Chromatography
Purified mouse monoclonal IgMκ in buffer containing PBS without preservatives.
Format: Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆mab 84, monoclonal
species reactivityhuman
technique(s)flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
同位素/亚型IgMκ
NCBI登记号NP_001018121
UniProt登记号O00592
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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