Anti-LRRK1 Antibody, clone 12F2.1

产品说明

一般描述

Leucine-rich repeat serine/threonine-protein kinase 1 (UniProt: Q38SD2, Leucine-rich repeat kinase 1) is encoded by the LRRK1 (also known as KIAA1790) gene (Gene ID: 79705) in human. Leucine-rich repeat kinase 1 is a member of the ROCO family of protein kinases. Two isoforms of Leucine-rich repeat kinase 1 have been described that are generated by alternative splicing. It contains a ROC (Ras in complex proteins) domain together with a MAPKKK-like kinase domain and several protein protein interactions. It is reported to form a complex with activated EGFR via interaction with Grb2. Subsequently, it is internalized to early endosomes along with EGFR. Leucine-rich repeat kinase 1 also regulates EGFR transport from early to late endosomes and is involved in regulating the motility of EGF-containing early endosomes that is dependent on its kinase activity. Leucine-rich repeat kinase 1 is also reported to play a role in regulating bone mass and mice with defective gene are prone to osteoporosis, increased bone mineralization, and diminished bone resorption. (Ref.: Hanafusa, H et al. (2011). Nature Commun. 2, Article number: 158).

特异性

Clone 12F2.1 detects Leucine-rich repeat serine/threonine-protein kinase 1 (LRRK1) in human. It targets an epitope within 86 amino acids from the internal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 86 amino acids from the internal region of human Leucine-rich repeat serine/threonine-protein kinase 1.

应用

Anti-LRRK1, clone 12F2.1, Cat. No. MABN2268, is a highly specific mouse monoclonal antibody that detects Leucine-rich repeat serine/threonine-protein kinase 1 (LRRK1) and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected LRRK1 in human cerebral cortex, human testis, and human pancreatic cancer tissues.

质量

Evaluated by Western Blotting in human hypothalamus tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected LRRK1 in 10 µg of human hypothalamus tissue lysate.

目标描述

~200 kDa observed; 225.39 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质