产品说明
特异性
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins collagen and non-collagen extracellular matrix proteins to remove unwanted reactivities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI collagens or fibronectin. Non-specific cross reaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible.
免疫原
Full length human laminin. Immunization occurred after single-band purity was assessed by SDS-PAGE.
应用
Detect Laminin using this Anti-Laminin Antibody validated for use in ELISA, IP, WB, IH.
Immunoblot: 1:500-1:2000
ELISA: 1:4000-1:8000
Immunohistochemistry: 1:50-1:200
Immunoprecipitation: Condition must be determined by the end user
Optimal working dilutions must be determined by the end user.
外形
Affinity purified immunoglobulin. Liquid at 1 mg/mL in 0.125 M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 with 0.01% Sodium Azide.
Format: Purified
储存及稳定性
The undiluted antibody solution is stable for approximately 6 months stored between 2 and 8° C. For extended storage mix with an equal volume of glycerol, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity purified immunoglobulin |
克隆 | polyclonal |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI登记号 | NM_005559.2 |
UniProt登记号 | P25391 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |