产品说明
一般描述
Cyclin-dependent kinase inhibitor 2A (UniProt P42771; also known as CDK4I, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, MTS-1, p16-INK4, p16-INK4a, p16INK4A) is encoded by the CDKN2A (also known as CDKN2, MTS1) gene (Gene ID 1029) in human. The INK and CIP/KIP families of cyclin dependent kinase inhibitors (CDKis) protect cells from oncogenic signals-initiated cellular transformation. INK4a and INK4b (encoded by the CDKN2A and CDKN2B gene, respectively) constitute the INK4 subfamily of CDKis. INK4a and INK4b are induced by oncogenic Ras and are involved in cell cycle G1 arrest and cellular senescence. Inactivation of CDKN2A and CDKN2B are found in a wide variety of human malignancies and murine tumor models.
特异性
Clone 13H4.1 epitope lies within a region present in p16INK4a and the spliced isoforms 2 and 5 (UniProt P42771-1, P42771-2, and P42771-4 respectively), but absent from the spliced isoform 3 (p12; UniProt P42771-3), p14ARF/p19ARF or smARF (UniProt Q8N726-1 and Q8N726-2).
免疫原
GST-tagged recombinant human p16INK4a internal fragment.
应用
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected p16INK4a in human kidney and small intestine tissue sections.
Anti-INK4a (p16), clone 13H4.1 Antibody, Cat. No. MABE1451, is a highly specific mouse monoclonal antibody that targets INK4a (p16) and has been tested in Immunocytochemistry and Western Blotting.
Research Category
Epigenetics & Nuclear Function
质量
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected p16INK4a in 10 µg of HEK293 cell lysate.
目标描述
~16 kDa observed. 16.53 kDa (isoform 1; p16INK4a), 11.22 kDa (isoform 2), and 17.88 (isoform 5; p16gamma) calculated. Uncharacterized bands may be observed in some lysate(s).
外形
Protein G purified.
Format: Purified
Purified mouse monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 13H4.1, monoclonal |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
| 同位素/亚型 | IgG2bκ |
| NCBI登记号 | NP_000068 |
| UniProt登记号 | P42771 |
| 运输 | ambient |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
