产品说明
一般描述
Protein G purified mouse monoclonal antibody. Recognizes the ~47 kDa Hsp47 protein.
Recognizes the ~47 kDa Hsp47 protein.
Anti-Hsp47, mouse monoclonal, clone M16.10A1, recognizes the ~47 kDa Hsp47 protein in wide variety of species. It is validated for use in ELISA, EIA, WB, ICC, IF, IP, and IHC (paraffin sections).
免疫原
Rat
Hsp47 from L6 cells
包装
50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
ELISA (see comments)
Enzyme Immunoassay (see comments)
Immunoblotting (1 µg/ml, colorimetric)
Immunocytochemistry (1 µg/ml)
Immunofluorescence (5 µg/ml)
Immunoprecipitation (5 µg/ml)
Paraffin Sections (see comments)
警告
Toxicity: Standard Handling (A)
外形
In PBS, 50% glycerol, pH 7.2.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
3T3 cells, Human breast cancer tissue
其他说明
Kuroda, K., et al. 1998. J. Invest. Dermatol.111, 1023.
Nandan, D., et al. 1998. Exp. Cell Res.179, 289.
Clark, E.P., et al. 1991. J. Biol. Chem.266, 17230.
Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J.274, 793.
Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
Saga, S., et al. 1987. J. Cell Biol.105, 517.
Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.
This antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | M16.10A1, monoclonal |
| 形式 | liquid |
| 包含 | ≤0.1% sodium azide as preservative |
| species reactivity | bovine, monkey, mouse, human, guinea pig, canine, rat, sheep, porcine, chicken, hamster |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 同位素/亚型 | IgG2b |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
