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Anti-Glutamine Synthetase Antibody, clone 11A3.1

品牌
Sigma-Aldrich
货号
MABN1182
规格纯度
clone 11A3.1, from mouse
参考价格
5688.25 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Glutamine synthetase (EC 4.1.1.15, EC 6.3.1.2; UniProt P09606; also known as Glutamate-ammonia ligase, Glutamate decarboxylase, GS) is encoded by the Glul (also known as Glns) gene (Gene ID 24957) in rat species. Glutamine synthetase (GS) plays an essential role in nitrogen metabolism by catalyzing ATP-dependent condensation of glutamate and ammonia to form glutamine. Three classes of GS exist, class I enzymes (GSI) are specific to prokaryotes and exist as oligomers of 12 identical subunits, class II enzymes (GSII) exist as decamer of identical subunits in eukaryotes and in some bacteria, while class III enzymes (GSIII) function as a double-ringed dodecamer of identical chains found in bacteroides fragilis and in butyrivibrio fibrisolvens. GS is present predominantly in the brain, kidneys, and liver in mammals. In the brain, GS is found primarily in astrocytes, where it participates in the the detoxification of ammonia via assimilation and termination of neurotransmitter signals via recyclization. c-Myc promotes glutamine usage by upregulating glutaminase (GLS), which converts glutamine to glutamate in the TCA cycle, while in some cancer cells Myc-mediated thymine DNA glycosylase (TDG) transcription leads to GS upregulation due to TDG-dependent demethylation of the GS promoter. GS upregulation promotes cancer cell survival under glutamine limitation, while GS silencing decreases cancer cell proliferation and xenograft tumor growth.

特异性

Clone 11A3.1 targets an internal epitope within the C-terminal half of human and rat glutamine synthetase.

免疫原

GST-tagged recombinant rat glutamine synthetase internal fragment.

应用

Western Blotting Analysis: A 1:20,000 dilution of from a representative lot detected Glutamine synthetase in 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Glutamine synthetase in human cerebellum, cerebral cortex, and tonsil tissue sections.

Research Category
Neuroscience
Anti-Glutamine Synthetase, clone 11A3.1, Cat. No. MABN1182, is a highly specific mouse monoclonal antibody that targets Glutamine Synthetase, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

质量

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Glutamine synthetase in 10 µg of rat brain tissue lysate.

目标描述

~42 kDa observed. 42.06 kDa (human) and 42.27 kDa (rat) calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified.
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆11A3.1, monoclonal
species reactivityhuman, rat
technique(s)immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_058769
UniProt登记号P09606
运输ambient

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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