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Anti-Dishevelled-2 Antibody

品牌
Sigma-Aldrich
货号
AB5972
规格纯度
Chemicon®, from rabbit
参考价格
6022.61 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

DVL2 is a member of multigene family that also contains DVL1 and DVL3. The vertebrate dishevelled proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein.

特异性

Disheveiled-2 protein (dvl-2). By Western blot the antibody shows no cross reactivity with recombinant disheveiled-1 (dvl-1) or disheveiled-3 (dvl-3) protein. Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against disheveiled-2 protein.

免疫原

Synthetic peptide corresponding to amino acids 600-614 of human and mouse disheveiled-2 protein.

应用

Western blot: 1:1,000 using ECL. The antibody reacts with a protein of ~95kDa. Faint reactivity was seen in the dvl-1 and dvl-3 HEK293 cell lysates suggesting there may be endogenous dvl-2 present.

Immunohistochemistry: 1:1,000 using the avidin-biotin system.

Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.
Detect Dishevelled-2 using this Anti-Dishevelled-2 Antibody validated for use in WB, IH.
Research Sub Category
Hematopoietic Stem Cells

Neural Stem Cells
Research Category
Stem Cell Research

目标描述

80 kDa

外形

Partially purified immunoglobulin. Liquid in PBS, pH 7.4, containing 0.01M sodium azide.
Format: Purified

储存及稳定性

Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

分析说明

Control
SK-N-SH (human neuroblastoma) whole cell lysates, NIH 3T3 whole cell lysate, BT-20 (human mammary gland) cell lysate, FP (mouse embryonal carcinoma

testicular teratoma) cell lysate

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式purified immunoglobulin (Partially purified)
antibody product typeprimary antibodies
克隆polyclonal
species reactivityhuman, mouse
manufacturer/tradenameChemicon®
technique(s)immunohistochemistry: suitable
western blot: suitable
inputsample type hematopoietic stem cell(s)
sample type neural stem cell(s)
NCBI登记号NM_004422.2
UniProt登记号O14641
运输wet ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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