产品说明
一般描述
DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation. The protein acts as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 mutation is likely responsible for transformation in neuroblastomas, and the Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1 gene, thus DVL1 maybe important in these diseases as well.
特异性
Dishevelled-1 protein (dvl-1). By Western blot the antibody shows no cross reactivity with recombinant dishevelled -2 or dishevelled -3 protein Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against dishevelled -1 protein.
免疫原
Synthetic peptide corresponding to amino acids 557-571 of human dishevelled-1 protein.
应用
Research Category
Stem Cell Research
Anti-Dishevelled-1 Antibody is an antibody against Dishevelled-1 for use in WB.
Western blot: 1:500 using ECL. The antibody reacts with a protein of ~85kDa. Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).
Optimal working dilutions must be determined by end user.
Research Sub Category
Hematopoietic Stem Cells
Neural Stem Cells
目标描述
85 kDa
外形
Liquid in PBS, pH 7.4, containing 0.01M sodium azide.
储存及稳定性
Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.
分析说明
Control
SK-N-SH (neuroblastoma) whole cell lysates
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | western blot: suitable |
| input | sample type neural stem cell(s) sample type hematopoietic stem cell(s) |
| NCBI登记号 | NM_004421.2 NM_181870.1 NM_182779.3 |
| UniProt登记号 | O14640 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
