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Anti-Connexin 43 Antibody

品牌
Sigma-Aldrich
货号
AB1727
规格纯度
Chemicon®, from rabbit
参考价格
6190.47 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Mouse Connexin 43 is a 382 amino acid gap junction protein withmouse connexin

a predicted M.W. of ~43 kDa. It is prominently expressed in heart (see reviews: Kumar & Giula 1996; White et al. 1995; Evans 1994; Beyer et al. 1990).

特异性

Mouse Cx43 immunogenic peptide sequence is specific for Cx43 and no significant homology is seen with other connexins. The mouse Cx43 peptide sequence shows 100% conserved with rat and bovine, and 84% with chicken and human (16/19 aa) Cx43 (Beyer et al. 1985; Nicholson et al. 1985; John et al. 1991; Fishman et al. 1990).

免疫原

KLH-conjugated synthetic peptide
corresponding to amino acids 360-382 within
the C-terminus of mouse connexin 43.

应用

Research Category
Cell Structure
ELISA: 1:10,000-100,000 using 50 - 100 ng Cx43 control peptide per well.
Immunocytochemistry: not tested. It is recommended that the antibody be tried at 2-20μg/mL in formaldehyde fixed (Beyer et al. 1985; Nicholson et al. 1985; John et al. 1991; Fishman et al. 1990).
Immunoblotting: 1-10μg/mL using Chemiluminescence technique.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: A 1:50 dilution of this antibody detected Connexin 43 in sections from mouse heart tissue pretreated with Tris-EDTA buffer, pH 9.0. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection.
This Anti-Connexin 43 Antibody is validated for use in ELISA, IP, WB, IC for the detection of Connexin 43.
Research Sub Category
Adhesion (CAMs)

外形

Affinity-purified using peptide-Sepharose column chromatography and supplied in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl containing 0.05% sodium azide.

储存及稳定性

Store at 2-8°C for 1 year from date of receipt.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityrat, mouse, bovine
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号NM_000165.3
UniProt登记号P17302
运输wet ice
Gene Informationhuman ... GJA1(2697)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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