Anti-ApoB Antibody, clone 2G11

产品说明

一般描述

Apolipoprotein B-100 (UniProt: E9Q414; also known as Apo B-100) is encoded by the Apob gene (Gene ID: 238055) in murine species. Apolipoprotein B is a major protein constituent of chylomicrons. Apo B particles are made up of lipids and proteins and each Apo B is bordered by a phospholipid monolayer with an inner core composed of variable amounts of triglycerides and cholesterol esters. Apo B is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off. Two isoforms of Apo B are produced- Apo B-100 is synthesized in the liver and Apo B-48 is produced in the intestine. Apo B-100 contains the region that binds to the LDL receptor. It contains VLDL particles, VLDL remnant particles and LDL particles. On the other hand, the Apo B-48 particles are made of chylomicrons and chylomicron remnant particles. Generally, LDL particles account for approximately 90% of Apo B particles in plasma. Abnormal trapping of an Apo B is a major factor in the development of atherosclerotic lesions. When Apo B particles degrade cholesterol is retained with macrophages that induce inflammatory response. Further entry and entrapment of Apo B can promote cardiovascular pathology over time. (Ref.: Sniderman, A et al. (2010). Nat. Rev. Endocrinol. 6(6); 335-346).

特异性

Clone 2G11 detects Apolipoprotein B in Mouse, Rat, and Hamster plasma and tissues. It binds to ApoB-48 and ApoB-100, but not to ApoB-39.

免疫原

ApoB-containing lipoproteins isolated from Apoe -/- Apob 48/48 mice.

应用

Western Blotting Analysis: 4 µg/mL from a representative lot detected ApoB in 10 µg of rat liver and mouse liver tissue lysate.

Western Blotting Analysis: A representative lot detected ApoB in Western Blotting applications (Nguyen, A.T., et. al. (2006). Biochem Biophys Acta. 1761(2):182-5; Cheng, D., et. al. (2016). J Biol Chem. 291(45):23793-23803).

ELISA Analysis: A representative lot detected ApoB in ELISA applications (Nguyen, A.T., et. al. (2006). Biochem Biophys Acta. 1761(2):182-5).

Research Category
Signaling
Detects Apolipoprotein B using this mouse monoclonal Anti-ApoB, clone 2G11 , Cat. No. MABS2046, is a mouse monoclonal antibody that detects Apolipoprotein B in Mouse, Rat, and Hamster and is tested for use in ELISA and Western Blotting.

质量

Evaluated by Western Blotting in rat serum.

Western Blotting Analysis: 4 µg/mL of this antibody detected ApoB in 10 µg of rat serum.

目标描述

~260 kDa observed; 509.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质