产品说明
一般描述
MVH (also known as DDX4 or Vasa) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. MVH is mostly localized in testicular germ cells in the cytoplasm of spermatocytes. MVH is a high-copy extragenic suppressor gene. MVH is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility and in stem cell development.
特异性
This antibody specifically recognizes wild type MVH acetylated at Lys405, but not the Lys405 mutant.
免疫原
Epitope: Acetylated Lys405
KLH-conjugated linear peptide corresponding to mouse MVH acetylated at Lys405.
应用
Western Blot/Immunoprecipitation Analysis: A representative lot detected Flag-tagged acetyl-MVH (Lys405) in the presence of Myc-HAT/p46.
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected acetyl-MVH (Lys405) in mouse germ cells (I. Nagamori, Department of Pharmacology, University of California, Irvine).
Research Sub Category
Cell Cycle, DNA Replication & Repair
Research Category
Stem Cell Research
Epigenetics & Nuclear Function
Anti-acetyl-MVH (Lys405) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-MVH (Lys405) also known as Probable ATP-dependent RNA helicase DDX4, DEAD box protein & has been validated in DB, WB, ICC, PIA & IP.
质量
Evaluated by Dot Blot/Peptide Inhibition Assay in non-acetylated and acetylated MVH (Lys405).
Dot Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected acetyl-MVH (Lys405) peptide.
目标描述
76 kDa calculated
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Non-acetylated and acetylated MVH (Lys405)
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | mouse, pig, human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology), porcine (based on 100% sequence homology) |
| technique(s) | dot blot: suitable immunocytochemistry: suitable immunoprecipitation (IP): suitable inhibition assay: suitable (peptide) western blot: suitable |
| NCBI登记号 | NP_034159 |
| UniProt登记号 | Q61496 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
