产品说明
一般描述
Immunoaffinity purified mouse monoclonal antibody. Recognizes 5-methylcytosine.
This Anti-5-Methylcytosine Mouse mAb (162 33 D3) is validated for use in ELISA, FC, Frozen Sections, IF, Paraffin Sections, Radioimmunoassay, Southwestern Blot for the detection of 5-Methylcytosine.
Recognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells.
Specific
• Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications
• Flow Cytometry
• Frozen Sections
• Immunoblotting
• Immunofluorescence
• Paraffin Sections
Reliable
• Generates reproducible results.
• Clone 162 33 D3 has been used in over 50 publications.
免疫原
5-methylcytosine conjugated to ovalbumin
包装
50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
ELISA (0.1 g/ml)
Flow Cytometry (1 g/ml)
Frozen Sections (5-10 g/ml)
Immunoblotting (5 g/ml)
Immunofluorescence (1 g/ml)
Paraffin Sections (5-10 g/ml)
Radioimmunoassay (0.1 g/ml)
Southwestern Blot (5 g/ml)
警告
Toxicity: Standard Handling (A)
外形
In PBS, pH 7.4.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
NIH3T3 cells
其他说明
Clone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems.
Ehrlich, M. 2002. Oncogene21, 5400. (Review)
Widschwendter, M. and Jones, P.A. 2002. Oncogene21, 5462. (Review)
Barton, S.C., et al. 2001. Hum. Mol. Genet.10, 2983.
Piyathilake, C.J., et al. 2001. Hum. Pathol.32, 856.
Taddei, A., et al. 2001. Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000. Biotech. Histochem.75, 251.
De Capoa, A., et al. 1999. FASEB J.13, 89.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | 162 33 D3, monoclonal |
形式 | liquid |
不包含 | preservative |
species reactivity (predicted by homology) | all |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
同位素/亚型 | IgG1 |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |