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Anti-5-Methylcytosine Mouse mAb (162 33 D3)

品牌
Sigma-Aldrich
货号
NA81
规格纯度
liquid, clone 162 33 D3, Calbiochem®
参考价格
5688.25 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Immunoaffinity purified mouse monoclonal antibody. Recognizes 5-methylcytosine.
This Anti-5-Methylcytosine Mouse mAb (162 33 D3) is validated for use in ELISA, FC, Frozen Sections, IF, Paraffin Sections, Radioimmunoassay, Southwestern Blot for the detection of 5-Methylcytosine.
Recognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells.

Specific
Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications
Flow Cytometry
Frozen Sections
Immunoblotting
Immunofluorescence
Paraffin Sections

Reliable
Generates reproducible results.
Clone 162 33 D3 has been used in over 50 publications.

免疫原

5-methylcytosine conjugated to ovalbumin

包装

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用



ELISA (0.1 g/ml)
Flow Cytometry (1 g/ml)
Frozen Sections (5-10 g/ml)
Immunoblotting (5 g/ml)
Immunofluorescence (1 g/ml)
Paraffin Sections (5-10 g/ml)
Radioimmunoassay (0.1 g/ml)
Southwestern Blot (5 g/ml)

警告

Toxicity: Standard Handling (A)

外形

In PBS, pH 7.4.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
NIH3T3 cells

其他说明

Clone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems.
Ehrlich, M. 2002. Oncogene21, 5400. (Review)
Widschwendter, M. and Jones, P.A. 2002. Oncogene21, 5462. (Review)
Barton, S.C., et al. 2001. Hum. Mol. Genet.10, 2983.
Piyathilake, C.J., et al. 2001. Hum. Pathol.32, 856.
Taddei, A., et al. 2001. Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000. Biotech. Histochem.75, 251.
De Capoa, A., et al. 1999. FASEB J.13, 89.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
生物来源mouse
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆162 33 D3, monoclonal
形式liquid
不包含 preservative
species reactivity (predicted by homology)all
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型IgG1
运输wet ice
储存温度−20℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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