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AEC

品牌
Sigma-Aldrich
CAS
132-32-1
货号
152224
规格纯度
This AEC is validated for use in Immunoblotting and Immunohistochemistry.
参考价格
2503.14 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Precipitable peroxidase substrate for immunoblotting and immunohistochemical staining techniques supplied as a 50X concentrate (0.095 mol/l). To be used with AEC Substrate Buffer, Cat. No. 152226
Precipitable peroxidase substrate for immunoblotting and immunohistochemical staining techniques. Produces a red end product that is soluble in alcohol. When used in immunohistochemistry, aqueous mounting media, and counterstains must be used. Directions included for use in each application.

包装

10 ml in Alu drum

应用

Immunoblotting (see comments)

Immunohistochemistry (see comments)

警告

Toxicity: Toxic (F)

重悬

Discard if a red or brown precipitate is evident.

其他说明

Okano, K., et al. 1992. Rinsho Byori 40, 93.
Krenacs, T., et al. 1990. Histochem. J. 22, 530.
Stable at 4°C and 18-26°C. Functionality determined by performing an immunohistologic stain using the final product.



Suggested procedure for use of AEC in immunohistochemistry and immunoblotting:



1. Complete all required incubations with antibodies and HRP-labeled probes, and all necessary wash steps.

2.Prepare 1X AEC Substrate Buffer: Add 9 parts distilled water to 1 part 10X AEC Substrate Buffer, Cat. No. 152226 (0.5 M acetate buffer containing 88 mM H2O2). Mix well. The final pH of this solution will be ~5.0.

3. Prepare 1X AEC Substrate Solution: Add 1 part 50X AEC Concentrate to 50 parts 1X AEC Substrate Buffer (prepared in Step 2). Mix well.

4. Following the final wash, completely cover tissue sections with 1X AEC Substrate Solution (prepared in Step 3) and incubate 10-30 min at room temperature. The same conditions apply for immunoblotting. Note: Variables associated with assay conditions will dictate the proper reaction times.

5. After reaction is complete, wash tissue sections or blotting membranes thoroughly in distilled water.

6. If tissue sections require a counterstain, Mayer′s Hematoxylin followed by blueing in alkaline tap water or a 0.5% aqueous methyl green solution is recommended.

7. Mount tissue sections in a water-based mounting medium. Air-dry blots and store protected from light.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式C14H14N2
分子量210.27
MDL编号MFCD00004964

产品性质

质量水平100
形式solution
不包含 preservative
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
protect from light
颜色 yellow-brown to reddish
运输ambient
储存温度2-8℃
InChI1S/C14H14N2/c1-2-16-13-6-4-3-5-11(13)12-9-10(15)7-8-14(12)16/h3-9H,2,15H2,1H3
InChI keyOXEUETBFKVCRNP-UHFFFAOYSA-N

安全信息

象形图GHS08
警示用语:Danger
危险声明H350 - H361d
预防措施声明P201 - P202 - P280 - P308 + P313 - P405 - P501
危险分类Carc. 1B - Repr. 2
储存分类代码6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGKWGK 3

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