产品说明
一般描述
The Immobilon®-FL transfer membrane is hydrophobic polyvinylidene fluoride (PVDF) microporous membrane used for the transfer of proteins from a variety of gel matrices. This transfer membrane offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. The Immobilon®-FL membrane has a nominal pore size of 0.45 μm and offers optimal blotting for proteins with molecular weights greater than 20,000 Daltons.
应用
Immobilon®-FL membrane has been used in western blotting. It is also suitable for use in standard chemiluminescent or chromogenic detection.
特点和优势
Immobilon®-FL Membrane:
- The first transfer membrane designed for fluorescence applications Extremely low background improves the sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- compatible with standard blocking agents and buffers
- Ideal for multiplexing and chemifluorescence detections
法律信息
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
eCl@ss | 32031602 |
NACRES | NB.22 |
产品性质
物料 | PVDF membrane plain filter white filter |
质量水平 | 400 |
特点 | hydrophobic |
manufacturer/tradename | Immobilon® |
technique(s) | western blot: suitable |
过滤器长度 × 宽度 | 10 cm × 10 cm |
孔径 | 0.45 μm pore size |
容量 | 155 μg/cm2 adsorption capacity (insulin) 205 μg/cm2 adsorption capacity (BSA) 300 μg/cm2 adsorption capacity (goat IgG) |
相容性 | for use with Amido black for use with CPTS for use with Coomassie brilliant blue for use with Ponceau-S red |
detection method | chemiluminescent colorimetric fluorometric |
运输 | ambient |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |