产品说明
一般描述
The Immobilon®-FL transfer membrane has a nominal pore size of 0.45 μm and is made up of polyvinylidene fluoride (PVDF) microporous membrane for binding proteins transferred from a variety of gel matrices. This membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biiomolecules. The membrane exhibits very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. In addition, the Immobilon®-FL membrane can be used for standard chemiluminescent or chromogenic detection. The Immobilon®-FL is recommended for optimal blotting of proteins with molecular weights greater than 20,000.
应用
Immobilon®-FL PVDF Membrane has been used in western blotting.
特点和优势
Immobilon®-FL Membrane:
- Designed for optimal fluorescent immunodetection
- Extremely low background improves the sensitivity of all fluorescence detection protocols
- suitable for all commonly used fluorescent probes at all excitation and emission wavelengths
- compatible with standard blocking agents and buffers
- Ideal for multiplexing and chemifluorescence detection
法律信息
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| eCl@ss | 32031602 |
| NACRES | NB.22 |
产品性质
| 物料 | PVDF membrane plain filter white filter |
| 质量水平 | 400 |
| 特点 | hydrophobic |
| manufacturer/tradename | Immobilon® |
| technique(s) | western blot: suitable |
| 过滤器长度 × 宽度 | 20 cm × 20 cm |
| 孔径 | 0.45 μm pore size |
| 容量 | 155 μg/cm2 adsorption capacity (insulin) 205 μg/cm2 adsorption capacity (BSA) 300 μg/cm2 adsorption capacity (goat IgG) |
| 相容性 | for use with Amido black for use with CPTS for use with Coomassie brilliant blue for use with Ponceau-S red |
| detection method | chemiluminescent colorimetric fluorometric |
| 运输 | ambient |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
