产品说明
一般描述
The Immobilon®-FL transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 μm. It is designed for binding proteins transferred from a variety of gel matrices. The Immobilon®-FL transfer membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane offers optimal blotting for proteins with molecular weights greater than 20,000. The Immobilon®-FL membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. Immobilon®-FL membrane can also be used for standard chemiluminescent or chromogenic detection.
应用
Immobilon-FL PVDF Membrane has been used in western blotting.
特点和优势
Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications; Extremely low background enhances the sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at a wide range of excitation/emission wavelengths
- Best for multiplexing and chemifluorescence detections
- compatible with standard blocking agents and buffers
法律信息
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| eCl@ss | 32031602 |
| NACRES | NB.22 |
产品性质
| 物料 | PVDF membrane plain filter white filter |
| 质量水平 | 400 |
| 特点 | hydrophobic |
| manufacturer/tradename | Immobilon® |
| technique(s) | western blot: suitable |
| 过滤器长度 × 宽度 | 26.5 cm × 3.75 m |
| 孔径 | 0.45 μm pore size |
| 容量 | 155 μg/cm2 adsorption capacity (insulin) 205 μg/cm2 adsorption capacity (BSA) 300 μg/cm2 adsorption capacity (goat IgG) |
| 相容性 | for use with All fluorescence dyes for use with Amido black for use with CPTS for use with Coomassie brilliant blue for use with Ponceau-S red for use with Sypro<TMSYMBOL></TMSYMBOL> ruby |
| detection method | chemiluminescent colorimetric fluorometric |
| 运输 | ambient |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
