Anti-Pan Shc Antibody, clone 3A12.1

产品说明

一般描述

SHC-transforming protein 1 (UniProt P29353; also known as SH2 domain protein C1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1) is encoded by the SHC1 (also known as SHC, SHCA) gene (Gene ID 6464) in human. Originally identified as an SH2-containing proto-oncogene involved in growth factor signaling, Shc proteins function as adaptor molecules recruited by numerous receptors (e.g. integrins, GPCRs, and receptors for growth factors, antigens, cytokines, and hormones) for signling. In addition to the ubiquitously expressed Shc (a.k.a. ShcA by SHC1/SHCA gene), there exist also ShcB (by SHC2/SHCB gene) and ShcC (by SHC3/SHCC gene), which are predominantly expressed in neuronal cells. Alternative splicings result in six ShcA isoforms, including p66Shc, p52Shc, and p46Shc. All ShcA isoforms possess two distinct domains, PTB (a.a. 156-339) and SH2 (a.a. 488-579), that bind phosphotyrosine-containing sequences, as well as a central region (CH1; a.a. 340-487) that contains critical tyrosine phosphorylation sites. The phosphotyrosine-binding (PTB) domain is also referred to as phosphotyrosine-interaction domain or PID.

特异性

Clone 3A12.1 was raised against a C-terminal region sequence present in all six human SHC1 spliced isoforms reported by UniProt (P29353).

免疫原

GST-tagged recombinant human Shc C-terminal region fragment.

应用

Anti-Pan Shc Antibody, clone 3A12.1 is an antibody against Pan Shc for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Shc proteins in human prostate cancer, as well as in normal breast and tonsil tissue sections.

质量

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Shc proteins in 10 µg of SH-SY5Y cell lysate.

目标描述

~47-63 kDa observed. 62.82 kDa (isoform p66Shc), 51.61 kDa (isoform p52Shc), 46.67 kDa (isoform p46Shc), 40.42 kDa (isoform 5), 62.89 kDa (isoform 6), 51.68 kDa (isoform 7) calculated.

外形

Protein G purified.
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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